细胞培养基 >> 细胞株
AsPC-1人转移胰腺腺癌细胞(2021年新引进)
产品介绍 目录号
Serial | SCSP-5080 | 标识符
Identifier | CSTR:19375.09.3101HUMSCSP5080 | 描述
Description | 该细胞系来源于人胰腺癌病人腹水中的细胞裸鼠异种移植而产生的癌性腹水细胞；该细胞可以表达CEA、人胰腺相关抗原、人胰腺特异性抗原和黏蛋白。
细胞质量控制方面：
1.支原体检测结果：本批次已通过本库支原体检测，结果为阴性；
2.STR鉴定结果：
Amelogenin: X
CSF1PO: 10,13
D13S317: 9,12
D16S539: 11
D5S818: 12
D7S820: 12,13
THO1: 7,9.3
TPOX: 8,10
vWA: 17 | 动物种别
Organism | 人，白人 | 性别
Gender | 女性，62岁 | 组织来源
Tissue and Cell Type | 胰腺；转移灶；腹水腺癌 | 形态
Morphology | 上皮细胞；贴壁生长 | 培养基和添加剂
Complete Growth Medium and Culture Conditions | 人转移胰腺腺癌细胞AsPC-1完全培养液配方(100 ml)：
RPMI-1640 (Invitrogen, 11875093)88 ml
FBS (Gibco)10 ml
Glutamax (Invitrogen, 35050061) 1 ml
Sodium Pyruvate 100 mM Solution (Invitrogen, 11360070) 1 ml
该细胞对血清质量敏感，我库建议您使用进口大品牌优质血清进行培养，如遇细胞死亡较多或生长缓慢，请更换血清品牌或批号。
参考传代比例：1:3-1:4
参考传代周期：3-5天
参考换液频率：2-3天
冻存液配方：完全培养液55%，FBS 40%，DMSO 5%
气相：空气，95%；二氧化碳，5%。温度：37摄氏度。 | 供应限制
Permits and Restrictions | A。仅供研究之用。 | | REFERENCE | Chen WH, et al. Human pancreatic adenocarcinoma: in vitro and in vivo morphology of a new tumor line established from ascites. In Vitro 18: 24-34, 1982.PubMed:7182348Tan MH, et al. Differential localization of human pancreas cancer-associated antigen and carcinoembryonic antigen in homologous pancreatic tumoral xenograft. J. Natl. Cancer Inst. 67: 563-569, 1981.PubMed:6944528Loor R, et al. Use of pancreas-specific antigen in immunodiagnosis of pancreatic cancer. Clin. Lab. Med. 2: 567-578, 1982.PubMed:6754226Lan MS, et al. Polypeptide core of a human pancreatic tumor mucin antigen. Cancer Res. 50: 2997-3001, 1990.PubMed:2334903Tan MH, Chu TM. Characterization of the tumorigenic and metastatic properties of a human pancreatic tumor cell line (AsPC-1) implanted orthotopically into nude mice. Tumor Biol. 6: 89-98, 1985.PubMed:4023565 |
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