试剂仪器设备原料
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细胞培养基 >> 细胞株
CCRF-CEM人急性淋巴细胞白血病T淋巴细胞
CCRF-CEM人急性淋巴细胞白血病T淋巴细胞图片
产品别名:CCRF CEM人急性淋巴细胞白血病T淋巴细胞
产品介绍
目录号
Serial
TCHu147
标识符
Identifier
CSTR:19375.09.3101HUMTCHu147
描述
Description
G.E. Foley 等人建立了类淋巴母细胞细胞株CCRF-CEM。 细胞是1964年11月从一位四岁白人女性急性淋巴细胞白血病患者的外周血白血球衣中得到。
在本库通过支原体检测。
在本库通过STR检测。
动物种别
Organism
性别
Gender
组织来源
Tissue and Cell Type
白血病, 急性淋巴细胞白血病, 外周血, T淋巴母细胞
形态
Morphology
淋巴母细胞
培养基和添加剂
Complete Growth Medium and Culture Conditions
RPMI 1640培养基(GIBCO by life technologies ,货号C11875500BT),90%;优质胎牛血清,10%。
气相:空气,95%;二氧化碳,5%。
温度:37摄氏度。
供应限制
Permits and Restrictions
A。仅供研究之用。
REFERENCEFoley GE et al. Continous culture of human lymphoblasts from peripheral blood of a child with acute leukemia. Cancer 18: 522-529, 1965    Sandstrom PA and Buttke TM. Autocrine production of extracellular catalase prevents apoptosis of the human CEM T-cell line in serum-free medium. Proc. Natl Acad. Sci. USA 90:4708-4712, 1993 PubMed: 93281648    Miranda L et al. Isolation of the human PC6 gene encoding the putative host protease for HIV-1 gpl60 processing in CD4+ T lymphocytes. Proc. Natl. Acad. Sci. USA 93: 7695-7700, 1996 PubMed: 96353880    Adams RA. Formal discussion: the role of transplantation in the  experimental  investigation  of  human  leukemia  and lymphoma. Cancer Res. 27: 2479-2482, 1967 PubMed: 68134846    Uzman BG et ah Morphologic variations in human leukemic lymphoblasts (CCRF-CEM cells) after long-term culture and exposure to chemotherapeutic agents. A study with the electron microscope. Cancer 19:1725-I742, 1966 PubMed: 67(146473    Adams RA et al. Leukemia: serial transplantation of human leukemic lymphoblasts in the newborn Syrian halnster. Cancer Res. 27: 772-783. 1967 PubMed: 68122005    Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2~a edition, Published by ATCC.    Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1 q88.    Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D.,(1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.    Centers   for  Disease   Control   (1993),   Biosafety   in Microbiological and Biomedical Laboratories Human Health Service Publication No. (CDC) 93-8395. U.S Dept. of Health and Human Services; 3rd Edition U.S. Government Printing Office Washington D.C.
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