产品介绍 目录号
Serial | TCHu140 | 标识符
Identifier | CSTR:19375.09.3101HUMTCHu140 | 描述
Description | 1967年五月E. Klein and G. Klein从一位16岁黑人男性Burkitt's淋巴瘤患者建立了Daudi细胞株。
表面免疫球蛋白阳性(sIg+)。Β2-微球蛋白阴性，EBNA阳性，并有衣壳抗原VCA。细胞株携带EB病毒。
Daudi是典型的B淋巴母细胞，广泛应用于白血病发生机理的研究。
在本库通过支原体检测。
在本库通过STR检测。 | 动物种别
Organism | 人 | 性别
Gender | 男 | 组织来源
Tissue and Cell Type | 外周血；B淋巴母细胞；Burkitt's淋巴瘤 | 形态
Morphology | 淋巴母细胞 | 培养基和添加剂
Complete Growth Medium and Culture Conditions | RPMI 1640培养基(GIBCO by life technologies ,货号C11875500BT)，90%；优质胎牛血清，10%。
气相：空气，95%；二氧化碳，5%。
温度：37摄氏度。 | 供应限制
Permits and Restrictions | A。仅供研究之用。 | | REFERENCE | Ohsugi Y , et al. Tumorigenicity of human malignant lymphoblasts: comparative study with unmanipulated nude mice, antilymphocyte serum-treated nude mice, and X- irradiated nude mice. J. Natl. Cancer Inst. 65: 715-718, 1980. PubMed: 6932523Klein E , et al. Surface IgM-kappa specificity on a Burkitt lymphoma cell in vivo and in derived culture lines. Cancer Res. 28: 1300-1310, 1968. PubMed: 4174339Huber C , et al. Surface receptors on human haematopoietic cell lines. Clin. Exp. Immunol. 25: 367-378, 1976. PubMed: 963908Nilsson K , et al. Tumorigenicity of human hematopoietic cell lines in athymic nude mice. Int. J. Cancer 19: 337-344, 1977. PubMed: 14896Gao Y , et al. Induction of an exceptionally high-level, nontranslated, Epstein-Barr virus-encoded polyadenylated transcript in the uurkitts lymphoma line Daudi. J. Virol. 71: 84-94, 1997. PubMed: 8985326Cuthbert JA , Lipsky PE . Regulation of proliferation and Ras localization in transformed cells by products of mevalonate metabolism. Cancer Res. 57: 3498-3504, 1997. PubMed: 9270019Yamaguchi Y , et al. Biochemical characterization and intracellular localization of the Menkes disease protein. Proc. Natl. Acad. Sci. USA 93: 14030-14035, 1996. PubMed: 8943055Lewis JA , et al. Inhibition of mitochondrial function by interferon. J. Biol. Chem. 271: 13184-13190, 1996. PubMed: 8662694Montoya JG , et al. Human CD4+ and CD8+ T lymphocytes are both cytotoxic to Toxoplasma gondii-infected cells. Infect. Immun. 64: 176-181, 1996. PubMed: 8557337 |
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