产品介绍 目录号
Serial | SCSP-5024 | 标识符
Identifier | CSTR:19375.09.3101HUMSCSP5024 | 描述
Description | 人前列腺癌细胞DU 145是从一位69岁白人前列腺癌患者的脑部转移病灶中建株的。该细胞株几乎检测不到激素敏感性，仅仅酸性磷酸酶呈弱阳性，分离得到的细胞在软琼脂中可形成克隆。该细胞不表达前列腺抗体。对该细胞株及原始肿瘤细胞的亚显微结构分析可见微绒毛、微丝及细胞桥粒、线粒体、发育良好的高尔基体和异质溶酶体。每个批次均通过本库支原体检测，结果为阴性。在我库通过STR鉴定无误。STR鉴定结果为：D7S820:7,10,11; D13S317:12,13,14; D5S818:10,13; vWA:17,18,19; THO1:7; CSF1PO:10,11; TPOX:11; D16S539:11,13; Amelogenin:X,Y | 动物种别
Organism | 人；白人 | 性别
Gender | 男性，69岁 | 组织来源
Tissue and Cell Type | 前列腺；来源于转移灶：大脑 | 形态
Morphology | 上皮样，贴壁生长 | 培养基和添加剂
Complete Growth Medium and Culture Conditions | 人前列腺癌细胞DU145完全培养液配方(100 ml)：
MEM (Invitrogen，11090081) 88 ml
FBS (Gibco)10 ml
Glutamax(invitrogen 35050061) 1 ml
Sodium Pyruvate 100 mM Solution(invitrogen 11360070) 1 ml
气相：空气，95%；二氧化碳，5%。温度：37摄氏度。 | 供应限制
Permits and Restrictions | A。仅供研究之用。 | | REFERENCE | Papsidero LD, et al. Prostate antigen: a marker for human prostate epithelial cells. J. Natl. Cancer Inst. 66: 37-42, 1981. PubMed: 6935463Stone KR, et al. Isolation of a human prostate carcinoma cell line (DU 145). Int. J. Cancer 21: 274-281, 1978. PubMed: 631930Mickey DD, et al. Heterotransplantation of a human prostatic adenocarcinoma cell line in nude mice. Cancer Res. 37: 4049-4058, 1977. PubMed: 908039Pollack MS, et al. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J. Natl. Cancer Inst. 66: 1003-1012, 1981. PubMed: 7017212Hu SX, et al. Development of an adenovirus vector with tetracycline-regulatable human tumor necrosis factor alpha gene expression. Cancer Res. 57: 3339-3343, 1997. PubMed: 9269991Sheng S, et al. Maspin acts at the cell membrane to inhibit invasion and motility of mammary and prostatic cancer cells. Proc. Natl. Acad. Sci. USA 93: 11669-11674, 1996. PubMed:8876194Carter RE, et al. Prostate-specific membrane antigen is a hydrolase with substrate and pharmacologic characteristics of a neuropeptidase. Proc. Natl. Acad. Sci. USA 93: 749-753, 1996. PubMed: 8570628Nupponen NN, et al. Genetic alterations in prostate cancer cell lines detected by comparative genomic hybridization. Cancer Genet. Cytogenet. 101: 53-57, 1998. PubMed: 9460501Robinson D, et al. A tyrosine kinase profile of prostate carcinoma. Proc. Natl. Acad. Sci. USA 93: 5958-5962, 1996. PubMed:8650201Su ZZ, et al. Surface-epitope masking and expression cloning identifies the human prostate carcinoma tumor antigen gene PCTA-1 a member of the galectin gene family. Proc. Natl. Acad. Sci. USA 93: 7252-7257, 1996. PubMed: 8692978Zhu X, et al. Cell cycle-dependent modulation of telomerase activity in tumor cells. Proc. Natl. Acad. Sci. USA 93: 6091-6095, 1996. PubMed: 8650224Boffa LC, et al. Invasion of the CAG triplet repeats by a complementary peptide nucleic acid inhibits transcription of the androgen receptor and TATA-binding protein genes and correlates with refolding of an active nucleosome containing a unique AR gene sequence. J. Biol. Chem. 271: 13228-13233, 1996. PubMed:8662737DU 145 was isolated by K.R. Stone et al from a lesion in the brain of a patient with metastatic carcinoma of the prostate and a 3 year history of lymphocytic leukemia. |
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