产品介绍 目录号
Serial | SCSP-5043 | 标识符
Identifier | CSTR:19375.09.3101HUMSCSP5043 | 描述
Description | MDA-MB-231来自患有转移乳腺腺癌的51岁白种人女病人的胸水。在裸鼠和ALS处理的BALB/c小鼠中，它能形成低分化腺癌(III级)。细胞表达WNT7B致癌基因。此细胞株可能作为转染宿主。我库冻存时为P41代。每个批次均通过本库支原体检测，结果阴性。经本库STR检测无误，STR鉴定结果为：Amelogenin: X；CSF1PO: 12,13；D13S317: 13；D16S539: 12；D5S818: 12；D7S820: 8,9；THO1: 7,9.3；TPOX: 8,9；vWA: 15,18。 | 动物种别
Organism | 人；白人 | 性别
Gender | 女性，51岁 | 组织来源
Tissue and Cell Type | 乳腺/乳房；来自转移灶：胸腔积液 | 形态
Morphology | 上皮样，贴壁生长 | 培养基和添加剂
Complete Growth Medium and Culture Conditions | 人乳腺癌细胞MDA-MB-231完全培养液配方(100 ml)：
L-15 (Hyclone, SH30525.01 或Invitrogen 11415-064)90 ml
FBS (Gibco) 10 ml
气相：空气，100%。温度：37摄氏度。
参考传代比例：1：3
参考传代周期：4-6天
参考换液频率：每周2次
冻存液配方：完全培养液95%，DMSO 5%
【注意事项：】
1.该细胞使用的L-15基础培养液由磷酸盐和游离碱氨基酸代替了碳酸氢钠缓冲体系。 该培养基必须在没有CO2平衡的环境中培养细胞。不能在普通的5% CO2细胞培养箱中培养，培养液颜色会明显变黄。
2.细胞形态大部分为纺锤状，也有部分细胞呈圆形，存在少量悬浮细胞。
3.如果发现细胞生长极其缓慢，可提高血清含量至20%。
4. 我库冻存时，每支冻存管约含7×105细胞量，体积为500μl，预期存活率70%，建议复苏至1个T25培养瓶中。 | 供应限制
Permits and Restrictions | A。仅供研究之用。 | | REFERENCE | Brinkley BR , et al. Variations in cell form and cytoskeleton in human breast carcinoma cells in vitro. Cancer Res. 40: 3118-3129, 1980. PubMed: 7000337 Cruciger Q , et al. Morphological, biochemical and chromosomal characterization of breast tumor lines from pleural effusions. In Vitro 12: 331, 1976. Siciliano MJ , et al. Mutually exclusive genetic signatures of human breast tumor cell lines with a common chromosomal marker. Cancer Res. 39: 919-922, 1979. PubMed: 427779 Cailleau R , et al. Breast tumor cell lines from pleural effusions. J. Natl. Cancer Inst. 53: 661-674, 1974. PubMed: 4412247 Cailleau R , et al. Long-term human breast carcinoma cell lines of metastatic origin: preliminary characterization. In Vitro 14: 911-915, 1978. PubMed: 730202 Bates SE , et al. Expression of the transforming growth factor-alpha/epidermal growth factor receptor pathway in normal human breast epithelial cells. Endocrinology 126: 596-607, 1990. PubMed: 2294006 Dickstein B , et al. Increased epidermal growth factor receptor in an estrogen-responsive, adriamycin-resistant MCF-7 cell line. J. Cell. Physiol. 157: 110-118, 1993. PubMed: 8408230 Huguet EL , et al. Differential expression of human Wnt genes 2, 3, 4, and 7B in human breast cell lines and normal and disease states of human breast tissue. Cancer Res. 54: 2615-2621, 1994. PubMed: 8168088 Satya-Prakash KL , et al. Cytogenetic analysis on eight human breast tumor cell lines: high frequencies of 1q, 11q and HeLa-like marker chromosomes. Cancer Genet. Cytogenet. 3: 61-73, 1981. PubMed: 7272986 Katayose Y , et al. Promoting apoptosis: a novel activity associated with the Cyclin-dependent kinase inhibitor p27. Cancer Res. 57: 5441-5445, 1997. PubMed: 9407946 Littlewood-Evans AJ , et al. The osteoclast-associated protease cathepsin K is expressed in human breast carcinoma. Cancer Res. 57: 5386-5390, 1997. PubMed: 9393764 Sheng S , et al. Maspin acts at the cell membrane to inhibit invasion and motility of mammary and prostatic cancer cells. Proc. Natl. Acad. Sci. USA 93: 11669-11674, 1996. PubMed: 8876194 De Vincenzo R , et al. Antiproliferative activity of colchicine analogues on MDR-positive and MDR-negative human cancer cell lines. Anticancer Drug Des. 13: 19-33, 1998. PubMed: 9474240 Soker S , et al. Characterization of novel vascular endothelial growth factor (VEGF) receptors on tumor cells that bind VEGF165 via its exon 7-endoded domain. J. Biol. Chem. 271: 5761-5767, 1996. PubMed: 8621443 |
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