产品介绍 目录号
Serial | SCSP-5030 | 标识符
Identifier | CSTR:19375.09.3101HUMSCSP5030 | 描述
Description | U-2 OS细胞来源于15岁白人女性，U-2 OS细胞与WI-38共培养或CF检测SV40，RSV或腺病毒时未检测到病毒。1972年曾发现被支原体污染并已去除。在我库通过支原体检测为阴性。冻存时为P36代。经本库STR检测结果无误。STR结果如下：Amelogenin: X；CSF1PO: 13；D13S317: 13；D16S539: 11,12；D5S818: 11；D7S820: 11,12；THO1: 6,9.3；TPOX: 11,12；vWA: 14,18 | 动物种别
Organism | 人 | 性别
Gender | 女性，15岁 | 组织来源
Tissue and Cell Type | 骨 | 形态
Morphology | 上皮样，贴壁生长 | 培养基和添加剂
Complete Growth Medium and Culture Conditions | 人骨肉瘤细胞U-2 OS完全培养液配方(100 ml)：
McCoy's 5a Medium (Invitrogen, 12330-031) 90 ml
FBS (Gibco) 10 ml | 供应限制
Permits and Restrictions | A。仅供研究之用。 | | REFERENCE | Heldin CH, et al. A human osteosarcoma cell line secretes a growth factor structurally related to a homodimer of PDGF A-chains. Nature 319: 511-514, 1986. PubMed: 3456080Ponten J, Saksela E. Two established in vitro cell lines from human mesenchymal tumours. Int. J. Cancer 2: 434-447, 1967. PubMed: 6081590Raile K, et al. Human osteosarcoma (U-2 OS) cells express both insulin-like growth factor-I (IGF-I) receptors and insulin-like growth factor-II/mannose-6- phosphate (IGF-II/M6P) receptors and synthesize IGF-II: autocrine growth stimulation by IGF-II via the IGF-I receptor. J. Cell. Physiol. 159 : 531-541 , 1994. PubMed: 8188767Landers JE, et al. Translational enhancement of mdm2 oncogene expression in human tumor cells containing a stabilized wild-type p53 protein. Cancer Res. 57: 3562-3568, 1997. PubMed: 9270029Moradpour D, et al. Characterization of cell lines allowing titghtly regulated expression of heapatitis C virus core protein. Virology 222: 51-63, 1996. PubMed: 8806487Ponten J, Saksela E. Two established in vitro cell lines from human mesenchymal tumours. Int. J. Cancer 2: 434-447, 1967. PubMed: 6081590 |
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