产品介绍 目录号
Serial | TCM34 | 标识符
Identifier | CSTR:19375.09.3101MOUTCM34 | 描述
Description | 这株细胞来源于转基因小鼠中生长的一个胰肿瘤(胰岛素瘤)。 这种小鼠携带了大鼠胰岛素II基因启动子调控的SV40早期基因的假基因结构。 细胞包含丰富的胰岛素和小量的胰高血糖素及生长抑素。 响应葡萄糖而分泌胰岛素[PubMed:7533732]。
在本库通过支原体检测。 | 动物种别
Organism | 小鼠 | 性别
Gender | *** | 组织来源
Tissue and Cell Type | 胰；胰岛素瘤；β细胞 | 形态
Morphology | 上皮细胞样 | 培养基和添加剂
Complete Growth Medium and Culture Conditions | DMEM培养基(GIBCO by life technologies ,货号C11995500BT)，85%；优质热灭活胎牛血清，15%。
气相：空气，95%；二氧化碳，5%。
温度：37摄氏度。 | 供应限制
Permits and Restrictions | A。仅供研究之用。 | | REFERENCE | Laurance ME , et al. Glucose responsive insulin secreting beta-cell lines and method for producing same. US Patent 5,773,255 dated Jun 30 1998Poitout V , et al. Morphological and functional characterization of beta TC-6 cells--an insulin-secreting cell line derived from transgenic mice. Diabetes 44: 306-313, 1995. PubMed: 7533732Poitout V , et al. Insulin-secreting cell lines: classification, characteristics and potential applications. Diabetes Metab. 22: 7-14, 1996. PubMed: 8697299Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.Centers for Disease Control (1993), Biosafety in Microbiological and Biomedical Laboratories Human Health Service Publication No. (CDC) 93-8395. U.S. Dept. of Health and Human Services; 3rd Edition U.S. Government Printing Office Washington D.C. |
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