试剂仪器设备原料
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细胞培养基 >> 细胞株
EL4小鼠淋巴瘤细胞
EL4小鼠淋巴瘤细胞图片
产品介绍
目录号
Serial
TCM41
标识符
Identifier
CSTR:19375.09.3101MOUTCM41
描述
Description
EL4是从用9,10-二甲基-1,2-苯并蒽在C57BL小鼠中诱导的淋巴瘤中建立的。 能抗0.1 mM 氢化可的松,对20 mcg/ml PHA敏感。 还有一个亚株(EL4.IL-2)可以生成高水平的IL-2。 检测表明肢骨发育畸形病毒(鼠痘)阴性。
在本库通过支原体检测。
动物种别
Organism
小鼠
性别
Gender
***
组织来源
Tissue and Cell Type
T 淋巴细胞;淋巴瘤
形态
Morphology
淋巴母细胞
培养基和添加剂
Complete Growth Medium and Culture Conditions
DMEM培养基(GIBCO by life technologies ,货号C11995500BT),90%;马血清,10%。
气相:空气,95%;二氧化碳,5%。
温度:37摄氏度。
供应限制
Permits and Restrictions
A。仅供研究之用。
REFERENCERalph P. Retention of lymphocyte characteristics by myelomas and theta+-lymphomas: sensitivity to cortisol and phytohemagglutinin. J. Immunol. 110: 1470-1475, 1973. PubMed: 4541304 Old LJ, et al. The G (Gross) leukemia antigen. Cancer Res. 25: 813-819, 1965. PubMed: 4284252 Gorer PA. Studies in antibody response of mice to tumour inoculation. Br. J. Cancer 4: 372-379, 1950. PubMed: 14801344 Herberman RB. Serological analysis of cell surface antigens of tumors induced by murine leukemia virus. J. Natl. Cancer Inst. 48: 265-271, 1972. PubMed: 4119883 Ralph P, Nakoinz I. Inhibitory effects of lectins and lymphocyte mitogens on murine lymphomas and myelomas. J. Natl. Cancer Inst. 51: 883-890, 1973. PubMed: 4542714 Shevach EM, et al. Immunoglobulin and theta-bearing murine leukemias and lymphomas. J. Immunol. 108: 1146-1151, 1972. PubMed: 4112916 Aparicio CL, et al. Correction for label leakage in fluorimetric assays of cell adhesion. BioTechniques 23: 1056-1060, 1997. PubMed: 9421636 Cuthbert JA, Lipsky PE. Regulation of proliferation and Ras localization in transformed cells by products of mevalonate metabolism. Cancer Res. 57: 3498-3504, 1997. PubMed: 9270019
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