产品介绍 目录号 Serial | GNR 4 | 标识符 Identifier | CSTR:19375.09.3101RATGNR4 | 描述 Description | L6成肌细胞株是Yaffe从甲基胆蒽存在下大鼠大腿肌的原代培养物最初两代中分离得到。 L6细胞在培养基中融合形成多核的肌管和横纹肌纤维。 细胞融合的程度随着代数的增加而下降,因而细胞应冷冻于低代次阶段并周期性地重新克隆以选择融合能力强的细胞。 如果让培养容器中的细胞长满,这株细胞的成肌组分将迅速耗尽。 检测表明肢骨发育畸形病毒(鼠痘)阴性。 在本库通过支原体检测。 | 动物种别 Organism | 大鼠 | 性别 Gender | *** | 组织来源 Tissue and Cell Type | 骨胳肌;成肌细胞 | 形态 Morphology | 成肌细胞 | 培养基和添加剂 Complete Growth Medium and Culture Conditions | DMEM培养基(GIBCO by life technologies ,货号C11995500BT),90%;优质胎牛血清,10%。 气相:空气,95%;二氧化碳,5%。 温度:37摄氏度。 | 供应限制 Permits and Restrictions | A。仅供研究之用。 | REFERENCE | Mandel JL , Pearson ML . Insulin stimulates myogenesis in a rat myoblast line. Nature 251: 618-620, 1974. PubMed: 4421831Richler C , Yaffe D . The in vitro cultivation and differentiation capacities of myogenic cell lines. Dev. Biol. 23: 1-22, 1970. PubMed: 5481965Yaffe D . Retention of differentiation potentialities during prolonged cultivation of myogenic cells. Proc. Natl. Acad. Sci. USA 61: 477-483, 1968. PubMed: 5245982Osawa H , et al. Identification and characterization of basal and cyclic AMP response elements in the promoter of the rat hexokinase II gene. J. Biol. Chem. 271: 17296-17303, 1996. PubMed: 8663388Osawa H , et al. Analysis of the signaling pathway involved in the regulation of hexokinase II gene transcription by insulin. J. Biol. Chem. 271: 16690-16694, 1996. PubMed: 8663315 |
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