质粒名称:GPD-roGFP
质粒编号:49436
质粒备注:Expresses thiol redox-sensitive ratiometric sensor roGFP in the mitochondrial intermembrane space of mammalian cells
载体名称:VQ Ad5CMV K-NpA
载体大小:5217
完整质粒大小:6200
载体类型:Mammalian Expression, Adenoviral
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:NEB Stable
高拷贝/低拷贝:高拷贝
插入基因:GPD-roGFP2
基因别名:IMS-roGFP
物种来源:Synthetic
基因突变:C48S, Q80R, S147C, and Q204C in Clontech's GFP
插入大小:3150
启动子:CMV
克隆方法:限制性内切酶
5'克隆位点:XhoI (not destroyed)
3'克隆位点:BamHI (not destroyed)
5'测序引物:5'GGCGTGTACGGTGGGAGGTCTATA-3'
3'测序引物:5'-TTAAAAAACCTCCCCACCTCCCCC-3'
质粒图谱: