质粒名称:pAC152-dual-dCas9VP64-sgExpression
质粒编号:48238
质粒备注:Dual expression construct expressing both dCas9VP64 and sgRNA from separate promoters
载体名称:pX335 (Addgene #42335)
载体类型:哺乳动物表达,CRISPR
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:DH5alpha
高拷贝/低拷贝:未知
插入基因:dCas9
物种来源:Synthetic
基因突变:D10A;H840A
插入大小:4349
标签/融合蛋白:· VP64 (C terminal on insert)
· HA Tag (N terminal on insert)
质粒图谱: