质粒名称:pBSFI-myr-akt-T308A/S473A
质粒编号:49191
质粒备注:cDNA of Akt1 with myristyolation signal and point mutation from T to A at position 308 and S to A at position 473 in cloning vector
载体名称:pBSFI
载体大小:3000
完整质粒大小:4500
载体类型:Adaptor Plasmid
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:DH5alpha
高拷贝/低拷贝:高拷贝
插入基因:Akt1
基因别名:Akt
物种来源:M. musculus (mouse)
基因突变:T308A; S473A
插入大小:1500
基因名称:Akt1 (a.k.a. Akt, LTR-akt, PKB, PKB/Akt, PKBalpha, Rac)
启动子:T7
标签/融合蛋白:· myristolation signal (N terminal on insert)
· HA tag (C terminal on insert)
克隆方法:限制性内切酶
5'克隆位点:BamHI (destroyed during cloning)
3'克隆位点:EcoRI (not destroyed)
5'测序引物:T3
3'测序引物:T7
质粒图谱: