质粒名称:pC-GoldyTALEN
质粒编号:38143
质粒备注:destination vector for the Golden Gate TALEN kit, directs expression of TALENs from a truncated CAGs promoter
载体名称:pBSK
载体大小:4000
完整质粒大小:5702
载体类型:哺乳动物表达
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:Top10
高拷贝/低拷贝:高拷贝
插入基因:GoldyTALEN
基因别名:TALEN
物种来源:Synthetic
基因突变:Truncate at N and C terminous
插入大小:1800
启动子:miniCaggs
标签/融合蛋白:· FokI homodimer (C terminal on insert)
· AcV5 (N terminal on insert)
克隆方法:限制性内切酶
5'克隆位点:Esp31 (destroyed during cloning)
3'克隆位点:Esp31 (destroyed during cloning)
5'测序引物:CGTGACCGCAATGGAGGC
3'测序引物:CACTGCATCCATGGCAGG
质粒图谱: