质粒名称:pCMV-ABE6.3
质粒编号:102916
质粒备注:expresses ABE6.3 in mammalian cells
载体名称:pBR322/CMV
载体大小:3388
完整质粒大小:8727
载体类型:哺乳动物表达
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:DH5alpha
高拷贝/低拷贝:高拷贝
插入基因:ABE6.3
基因别名:TadA-linker(32aa)-TadA*(6.3)-linker(32aa)-nCas9-NLS
物种来源:Synthetic; TadA is from E.coli (synthetic gene block, mammalian codon optimized), Cas9 is S. pyogenes (mammalian codon optimized)
基因突变:see manuscript
插入大小:5328
启动子:CMV
克隆方法:Ligation Independent Cloning
5'测序引物:T7
3'测序引物:M13
质粒图谱: