质粒名称:pdCas9-bacteria
质粒编号:44249
质粒备注:aTc-inducible expression of a catalytically inactive bacterial Cas9 (S. pyogenes) for bacterial gene knockdown
载体名称:p15A vector
完整质粒大小:6705
载体类型:Bacterial Expression, CRISPR
原核抗性:氯霉素25μg/mL
培养温度:37℃
克隆菌株:Top10
高拷贝/低拷贝:低拷贝
插入基因:dCas9 (bacteria)
基因别名:Catalytically inactive Cas9
物种来源:S. pyogenes
基因突变:D10A H840A (catalytically inactive)
插入大小:4107
启动子:pLtetO-1
克隆方法:限制性内切酶
5'克隆位点:BglII (not destroyed)
3'克隆位点:XhoI (not destroyed)
5'测序引物:ttcaaaaGATCTAAAGAGGAGAAAGGATCTATGGATAAGAAATACTCAATAG
3'测序引物:tgcctggagatccttactcgaGTTAGTCACCTCCTAGCTGACTCAAATCAAT
质粒图谱: