质粒名称:pdCas9-humanized
质粒编号:44246
质粒备注:Expression of a catalytically inactive, human codon-optimized Cas9 under the control of Murine Stem Cell retroVirus LTR promoter for mammalian gene knockdown
载体名称:pMSCVpuro
完整质粒大小:10477
载体类型:Mammalian Expression, Retroviral, CRISPR ; vector
筛选标记:嘌呤霉素
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:DH5alpha
高拷贝/低拷贝:高拷贝
插入基因:dead Cas9 with 3X NLS
基因别名:Catalytically inactive Cas9
物种来源:H. sapiens (human)
基因突变:D10A H840A (catalytically inactive)
插入大小:4188
启动子:MSCV LTR promoter
克隆方法:未知
5'测序引物:CCGGAATTAGATCTCGCCACCATGGAC
3'测序引物:CCGGTAGAATTCTATACCTTTCTCTTCT
质粒图谱: