质粒名称:pET28a-MH6-RspCas13d
质粒编号:108305
质粒备注:Expresses E. coli codon optimized RspCas13d in the pET28a backbone.
载体名称:pET-28a(+)
载体大小:5390
完整质粒大小:8153
载体类型:Bacterial Expression, CRISPR
原核抗性:卡那霉素,50μg/mL
培养温度:37℃
克隆菌株:DH5alpha
高拷贝/低拷贝:低拷贝
插入基因:RspCas13d
物种来源:Synthetic
插入大小:2763
启动子:lac
克隆方法:限制性内切酶
5'克隆位点:EcoRI (not destroyed)
3'克隆位点:EcoRI (not destroyed)
5'测序引物:ATGGCCAAAAAGAACAAAATGAAACC
质粒图谱: