质粒名称:pFA6a-3HA-kanMX6
质粒编号:39295
载体名称:pFA6a
载体大小:2300
载体类型:Yeast genomic targeting
筛选标记:Gentamicin
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:DH5alpha
高拷贝/低拷贝:未知
插入基因:KanR
基因别名:kanamycin resistance cassette
插入大小:2000
标签/融合蛋白:· 3HA (N terminal on insert)
· yeast ADH1 terminator (N terminal on insert)
· A. gossypii translation elongation factor 1a gene promoter (N terminal on insert)
· A. gossypii translation elongation factor 1a gene terminator (C terminal on insert)
克隆方法:限制性内切酶
5'克隆位点:PacI (not destroyed)
3'克隆位点:PmeI (not destroyed)
5'测序引物:Sp6_primer
3'测序引物:T7_promoter
质粒图谱: