质粒名称:phage-ubc-nls-ha-tdMCP-gfp
质粒编号:40649
载体名称:pHAGE-UBC-RIG (modified)
载体类型:哺乳动物表达,慢病毒
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:DH5alpha
高拷贝/低拷贝:未知
插入基因:tdMCP-gfp
基因别名:tandem dimer MS2 coat protein (MCP)
物种来源:Synthetic
启动子:human ubiquitin C (UBC)
标签/融合蛋白:· NLS (N terminal on backbone)
· HA (N terminal on backbone)
· EGFP (C terminal on backbone)
克隆方法:限制性内切酶
5'克隆位点:unknown (unknown if destroyed)
3'克隆位点:unknown (unknown if destroyed)
5'测序引物:FUGW (5'-ATTACAGGGACAGCAGAGATCC-3')
3'测序引物:WPRE-R (5'CATAGCGTAAAAGGAGCAACA-3')
质粒图谱: