质粒名称:pHLmMBP-10
质粒编号:72348
质粒备注:(Empty Backbone) Mammalian expression of secreted N-terminally 8His-tagged mMBP TEV cleavage site-linked fusion proteins with C-terminal 6His-tag/Strep-tag II/HA-tag
载体名称:pHLmMBP-10
载体大小:5931
载体类型:哺乳动物表达
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:DH5alpha
高拷贝/低拷贝:高拷贝
启动子:CMV enhancer + chicken beta-actin promoter
标签/融合蛋白:· 8His-tag (N terminal on backbone)
· mMBP with C-terminal TEV cleavage site linker (N terminal on backbone)
· 6His-tag (C terminal on backbone)
· Strep-tag II (C terminal on backbone)
· HA-tag (C terminal on backbone)
克隆方法:限制性内切酶
5'测序引物:AAGGTGAAATCATGCCCAACATCC
3'测序引物:ATTTGTGAGCCAGGGCATTG
质粒图谱: