质粒名称:pHR-SFFV-dCas9-BFP-KRAB
质粒编号:46911
质粒备注:Human expression vector containing SFFV promoter, dCas9 that is fused to 2x NLS, tagBFP and a KRAB domain
载体名称:pHR
载体大小:9000
完整质粒大小:14167
载体类型:哺乳动物表达,慢病毒,CRISPR
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:Stbl3
高拷贝/低拷贝:高拷贝
插入基因:dCas9-BFP-KRAB fusion
物种来源:H. sapiens (human)
插入大小:5172
启动子:SFFV
标签/融合蛋白:· HA (C terminal on insert)
· 2xNLS (C terminal on insert)
· BFP (C terminal on insert)
· KRAB domain (C terminal on insert)
克隆方法:Ligation Independent Cloning
5'测序引物:gcttcccgagctctataaaagag
3'测序引物:CCAGAGGTTGATTATCGATAAGC
质粒图谱: