质粒名称:pHSN401
质粒编号:50588
质粒备注:CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance
载体名称:pGreen-like binary vector
载体类型:CRISPR ; Plant expression
筛选标记:潮霉素
原核抗性:Kanamycin and Spectinomycin, 50 & 50 μg/mL
培养温度:37℃
克隆菌株:DH5alpha
高拷贝/低拷贝:高拷贝
插入基因:3×FLAG-NLS-zCas9-NLS
基因别名:Cas9
物种来源:Synthetic
基因突变:maize codon optimized
启动子:2×35Sp
标签/融合蛋白:· 3xFLAG (N terminal on insert)
· NLS (N terminal on insert)
克隆方法:未知
5'测序引物:hSpCas9-R1, CGCTCGTGCTTCTTATCCTC
质粒图谱: