质粒名称:pJK142
质粒编号:73317
质粒备注:Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) as a fusion to Pas2p, with c-myc tag and His6-tag
载体名称:pPICZA
载体大小:3329
完整质粒大小:5856
载体类型:酵母表达
筛选标记:Zeocin
原核抗性:Bleocin (Zeocin), 50 μg/mL
培养温度:37℃
克隆菌株:DH5alpha
高拷贝/低拷贝:未知
插入基因:delta-12 fatty acid acetylenase
基因别名:vFAD2
物种来源:Crepis alpina
基因突变:synthetic gene. Encodes a glycine-204 to serine mutation in the Pas2 fusion domain
启动子:AOX1
标签/融合蛋白:· c-myc (C terminal on backbone)
· His6 (C terminal on backbone)
· Pas2p (N terminal on insert)
克隆方法:限制性内切酶
5'克隆位点:PmlI (not destroyed)
3'克隆位点:ApaI (not destroyed)
5'测序引物:5'-AOX1 primer
3'测序引物:3'-AOX primer
质粒图谱: