质粒名称:pLV312.3
质粒编号:119944
质粒备注:Expresses C-terminal Npu DnaE Intein-SaKKH-BE3, tagRFP, and sgRNA in mammalian cells
载体名称:px601
载体大小:4316
完整质粒大小:6516
载体类型:Mammalian Expression, AAV, CRISPR
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:NEB Stable
高拷贝/低拷贝:高拷贝
插入基因:C-Intein (Npu DnaE) - C-terminal (740)SaKKH-BE3
基因别名:split BE3 (C-terminus)
物种来源:R. norvegicus (rat); S. aureus
插入大小:3345
启动子:CMV
克隆方法:Gibson Cloning
5'测序引物:CGCAAATGGGCGGTAGGCGTG (CMV for)
3'测序引物:CCTCGACTGTGCCTTCTA (bGH rev)
质粒图谱: