质粒名称:pLV-mitoDsRed
质粒编号:44386
质粒备注:3rd generation lentiviral vector
载体名称:pLV
载体大小:6727
完整质粒大小:7600
载体类型:哺乳动物表达,慢病毒
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:Stbl3
高拷贝/低拷贝:高拷贝
插入基因:mitoDsRed
物种来源:H. sapiens (human)
插入大小:880
启动子:CMV
标签/融合蛋白:· 61 aa targeting sequence of the P1 isoform F1F0-ATP synthase (N terminal on insert)
· DsRed (C terminal on insert)
克隆方法:限制性内切酶
5'克隆位点:SpeI (destroyed during cloning)
3'克隆位点:EcoRV (destroyed during cloning)
5'测序引物:CMV-F
3'测序引物:WPRE-R
质粒图谱: