质粒名称:pML104
质粒编号:67638
质粒备注:Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains URA3 marker for yeast transformation
载体名称:pRSII426
载体大小:5718
完整质粒大小:11240
载体类型:Yeast Expression, CRISPR
筛选标记:URA3
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:DH5alpha
高拷贝/低拷贝:高拷贝
插入基因:Cas9
物种来源:S. cerevisiae (budding yeast); Streptococcus pyogenes
插入大小:4151
启动子:pTDH3
克隆方法:限制性内切酶
5'克隆位点:EcoRI (not destroyed)
3'克隆位点:XhoI (not destroyed)
5'测序引物:T7
质粒图谱: