质粒名称:pMSCV-LTR-dCas9-VP64-BFP
质粒编号:46912
质粒备注:Human expression vector containing MSCV LTR promoter, dCas9 that is fused to 2x NLS, VP64 and tagBFP
载体名称:MSCV-puro
载体大小:6300
完整质粒大小:11369
载体类型:Mammalian Expression, Retroviral, CRISPR
筛选标记:嘌呤霉素
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:Stbl3
高拷贝/低拷贝:低拷贝
插入基因:dCas9-VP64-BFP fusion
物种来源:H. sapiens (human)
插入大小:5055
启动子:LTR
标签/融合蛋白:· 3xNLS (C terminal on insert)
· BFP (C terminal on insert)
· VP64 domain (C terminal on insert)
克隆方法:Ligation Independent Cloning
5'测序引物:CCCTTGAACCTCCTCGTTCGACC
3'测序引物:GAGACGTGCTACTTCCATTTGTC
质粒图谱: