质粒名称:pMV306hsp+LuxG13
质粒编号:26161
载体名称:pMV306hsp
载体大小:4373
载体类型:原核表达
原核抗性:卡那霉素,50μg/mL
培养温度:37℃
克隆菌株:DH5alpha
高拷贝/低拷贝:低拷贝
插入基因:Bacterial luciferase operon + G13 promoter
基因别名:LuxABCDE
物种来源:Mycobacterium marinum + Photorhabdus luminescens
基因突变:It contains a gram-positive enhanced translation signal in front of luxA, luxC and luxE. G13 promoter cloned in front of luxC
插入大小:6186
克隆方法:限制性内切酶
5'克隆位点:EcoRI (not destroyed)
3'克隆位点:EcoRV (destroyed during cloning)
5'测序引物:agaataacgttggcactcgc
质粒图谱: