质粒名称:pRK5-HA-Ubiquitin-K63
质粒编号:17606
质粒备注:Mammalian expression of HA tagged ubiquitin with K63 only, other lysines mutated to arginines
载体名称:pRK5-HA
载体大小:4800
载体类型:哺乳动物表达
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:DH5alpha
高拷贝/低拷贝:高拷贝
插入基因:Ubiquitin C
基因别名:Ub
物种来源:H. sapiens (human)
基因突变:K63 only, other lysines mutated to arginines. Enhances G76-K63-linked polyubiquitination.
插入大小:220
基因名称:UBC (a.k.a. HMG20)
克隆方法:限制性内切酶
5'克隆位点:SalI (not destroyed)
3'克隆位点:NotI (not destroyed)
5'测序引物:SP6
质粒图谱: