质粒名称:pCAG-CBE4max-SpRY-P2A-EGFP (RTW5133)
质粒编号:139999
质粒备注:CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP
载体名称:pRZ33
载体类型:哺乳动物表达,CRISPR
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:DH5alpha
高拷贝/低拷贝:高拷贝
插入基因:human codon optimized CBE4max SpCas9 variant named SpRY with P2A-EGFP
基因别名:RTW5133
物种来源:Synthetic
基因突变:nSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R
启动子:CAG
克隆方法:Gibson Cloning
5'测序引物:oBK280-CCTACAGCTCCTGGGCAACG
3'测序引物:oBK23-CAGAGGGAAAAAGATCTCAGTGG
质粒图谱: