质粒名称:pFA6a-GFP(S65T)-TRP1
质粒编号:41597
载体名称:pFA6a
载体大小:2300
载体类型:Yeast genomic targeting
筛选标记:TRP1
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:DH5alpha
高拷贝/低拷贝:未知
插入基因:TRP1
物种来源:S. cerevisiae (budding yeast)
插入大小:1981
基因名称:TRP1 (a.k.a. YDR007W)
标签/融合蛋白:· GFP (S65T) (N terminal on insert)
· yeast ADH1 terminator (N terminal on insert)
克隆方法:限制性内切酶
5'克隆位点:PacI (not destroyed)
3'克隆位点:PmeI (not destroyed)
5'测序引物:Sp6_primer
3'测序引物:T7_promoter
质粒图谱: