质粒名称:pFA6a-His3MX6-PGAL1-3HA
质粒编号:41610
载体名称:pFA6a
载体类型:Yeast genomic targeting
筛选标记:HIS3
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:DH5alpha
高拷贝/低拷贝:未知
插入基因:GAL1 promoter
基因别名:GAL1
物种来源:S. cerevisiae (budding yeast)
插入大小:2045
基因名称:GAL1 (a.k.a. YBR020W)
标签/融合蛋白:· A. gossypii translation elongation factor 1a gene terminator (N terminal on insert)
· S. kluyveri His3 reverse orientation (N terminal on insert)
· A. gossypii translation elongation factor 1a gene promoter (N terminal on insert)
· 3xHA (C terminal on insert)
· yeast ADH1 terminator (C terminal on insert)
克隆方法:限制性内切酶
5'克隆位点:BglII (not destroyed)
3'克隆位点:PacI (not destroyed)
5'测序引物:T7_promoter
3'测序引物:Sp6_primer
质粒图谱: