质粒名称:pQLinkH
质粒编号:13667
载体名称:pQE-2
载体大小:4873
载体类型:Bacterial Expression ; Co-expression
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:DH5alpha
高拷贝/低拷贝:未知
插入基因:multi cloning site
基因别名:pQTEV3
插入大小:28
标签/融合蛋白:· His (N terminal on backbone)
· attB1 (N terminal on backbone)
· TEV (N terminal on backbone)
克隆方法:限制性内切酶
5'克隆位点:BamHI (not destroyed)
3'克隆位点:NotI (not destroyed)
5'测序引物:pQE65, TGAGCGGATAACAATTTCACACAG
3'测序引物:pQE276, GGCAACCGAGCGTTCTGAAC
质粒图谱: