质粒名称:PX552
质粒编号:60958
质粒备注:pAAV-U6sgRNA(SapI)_hSyn-GFP-KASH-bGH (SpGuide acceptor). AAV plasmid for sgRNA cloning. GFP-KASH fusion facilitates FACS sorting of cells and nuclei.
载体名称:pAAV
载体大小:2905
完整质粒大小:5314
载体类型:Mammalian Expression, Mouse Targeting, AAV, CRISPR
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:Stbl3
高拷贝/低拷贝:高拷贝
插入基因:U6_(SpaI)_sgRNA
物种来源:Synthetic
插入大小:351
启动子:U6
克隆方法:Gibson Cloning
5'测序引物:cgcgtgagggcctatttcc
3'测序引物:gcaagtgggttttaggaccag
质粒图谱: