质粒名称:lentiCRISPRv2 puro
质粒编号:98290
质粒备注:(Empty Backbone) Variant of lentiCRISPRv2 that confers puromycin resistance
载体名称:lentiCRISPRv2
载体大小:14877
载体类型:Lentiviral, CRISPR
筛选标记:嘌呤霉素
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:NEB Stable
高拷贝/低拷贝:高拷贝
插入基因:P2A-puro
启动子:EF-1a
克隆方法:限制性内切酶
5'克隆位点:BamHI (not destroyed)
3'克隆位点:MluI (not destroyed)
5'测序引物:CCAAAGAGGTGCTGGACG
3'测序引物:WPRE-R (CATAGCGTAAAAGGAGCAACA)
质粒图谱: