质粒名称:lentiGuide-Puro
质粒编号:52963
质粒备注:Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter. 3rd generation lentiviral backbone.
载体名称:Custom
完整质粒大小:10183
载体类型:哺乳动物表达,慢病毒,CRISPR
筛选标记:嘌呤霉素
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:NEB Stable
高拷贝/低拷贝:高拷贝
插入基因:S. pyogenes sgRNA cassette
基因别名:S. pyogenes CRISPR customizable RNA element
物种来源:Synthetic
插入大小:100
启动子:hU6
克隆方法:限制性内切酶
5'克隆位点:BsmBI (not destroyed)
3'克隆位点:BsmBI (not destroyed)
5'测序引物:hU6-F
3'测序引物:hGata4-rev (5'-ATTGTGGATGAATACTGCC-3')
质粒图谱: