质粒名称:pAAV_hsyn_NES-his-CaMPARI2-H396K-WPRE-SV40
质粒编号:101062
质粒备注:AAV vector expressing CaMPARI2_H396K (Kd = 360nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons
载体名称:pAAV
载体大小:4267
完整质粒大小:5716
载体类型:AAV
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:NEB Stable
高拷贝/低拷贝:高拷贝
插入基因:CaMPARI2-H396K
物种来源:Synthetic
插入大小:1446
启动子:hsyn (synapsin-1)
标签/融合蛋白:· NES_his (N terminal on insert)
· FLAG-HA-myc (C terminal on insert)
克隆方法:Gibson Cloning
5'测序引物:f.hsyn
3'测序引物:r.WPRE
质粒图谱: