质粒名称:pCAG-enAsCas12a-HF1(E174R/N282A/S542R/K548R)-NLS(nuc)-3xHA (AAS1815)
质粒编号:107942
质粒备注:Mammalian expression plasmid for human codon optimized enAsCas12a-HF1 (enhanced high-fidelity AsCas12a) encoding E174R/N282A/S542R/K548R substitutions
载体名称:pCAG-CFP
载体类型:哺乳动物表达
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:DH5alpha
高拷贝/低拷贝:高拷贝
插入基因:human codon optimized enAsCas12a-HF1 (E174R/N282A/S542R/K548R)
基因别名:AAS1815
基因别名:enhanced, high-fidelity version of Cas12a from Acidaminococcus sp. BV3L6
物种来源:Acidaminococcus sp. BV3L6
基因突变:E174R, S542R, K548R, and N282A
启动子:CAG
标签/融合蛋白:· NLS (nucleoplasmin) (C terminal on insert)
· 3x HA (C terminal on insert)
克隆方法:Gibson Cloning
5'测序引物:pCAG-F (5'-GCAACGTGCTGGTTATTGTG-3')
3'测序引物:Bglob-pA-R (5'-ttttggcagagggaaaaaga-3')
质粒图谱: