质粒名称:pEF1a-FB-dCas9-puro
质粒编号:100547
质粒备注:Expresses N-terminal Flag and biotin-acceptor-site (FB)-tagged dCas9 protein
载体名称:pEF1a-FB-puro
载体大小:7064
完整质粒大小:11244
载体类型:哺乳动物表达,CRISPR
筛选标记:嘌呤霉素
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:DH5alpha
高拷贝/低拷贝:未知
插入基因:N-terminal Flag and biotin-acceptor-site (FB)-tagged dCas9
物种来源:Streptococcus pyogenes
插入大小:4305
启动子:Human EF1a
克隆方法:Gibson Cloning
5'测序引物:CTTCCATTTCAGGTGTCGTG
3'测序引物:ctggccacctctgcttgt
质粒图谱: