质粒名称:pEJS578_DD-dSpyCas9-mCherry-APEX2
质粒编号:108570
质粒备注:Expression of drug-inducible dSpyCas9-mCherry-APEX2 for biotin-labeling genomic element-associated protein factors
载体名称:pHAGE-DEST
载体大小:6000
完整质粒大小:12774
载体类型:哺乳动物表达,慢病毒,CRISPR
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:NEB Stable
高拷贝/低拷贝:高拷贝
插入基因:dSpyCas9
物种来源:Synthetic
插入大小:4113
启动子:CMV
标签/融合蛋白:· Destabilization domains (N terminal on insert)
· mCherry (C terminal on insert)
· Flag (C terminal on insert)
· APEX2 (C terminal on insert)
克隆方法:限制性内切酶
5'克隆位点:MluI (not destroyed)
3'克隆位点:SpeI (not destroyed)
5'测序引物:cgcaaatgggcggtaggcgtg
质粒图谱: