质粒名称:pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE
质粒编号:104495
质粒备注:AAV-mediated expression of ultrasensitive protein calcium sensor under the CAG promoter, Cre mediated flip-excision switch
载体名称:AAV-CAG-FLEX
载体大小:5027
完整质粒大小:6380
载体类型:Mammalian Expression, AAV, Cre/Lox
原核抗性:氨苄青霉素,100μg/mL
培养温度:30℃
克隆菌株:NEB Stable
高拷贝/低拷贝:低拷贝
插入基因:jGCaMP7s
基因别名:GCaMP3-A52V K78H T302L R303P A317L D380Y T381R S383T R392G
基因别名:GCaMP3 variant 1473
基因别名:Janelia GCaMP7
物种来源:R. norvegicus (rat), G. gallus (chicken); A. victoria (jellyfish)
插入大小:1353
启动子:CAG
克隆方法:限制性内切酶
5'克隆位点:BsmBI (destroyed during cloning)
3'克隆位点:BsmBI (destroyed during cloning)
5'测序引物:GGTTCGGCTTCTGGCGTGTGACC
质粒图谱: