质粒名称:pK7WGF2::hCas9
质粒编号:46965
质粒备注:Expesses the human codon usage Cas9 nuclease (Mali et al. Science 339, 823-826, 2013) with an N-terminal GFP tag from the 35S promoter in the plant tissue
载体名称:pK7WGF2
载体大小:10257
完整质粒大小:14397
载体类型:CRISPR ; Plant expression
筛选标记:Kanamycin
原核抗性:Spectinomycin, 50 μg/mL
培养温度:37℃
克隆菌株:DH5alpha
高拷贝/低拷贝:低拷贝
插入基因:hCas9
物种来源:Synthetic
插入大小:4140
启动子:35S
克隆方法:Gateway Cloning
5'测序引物:CAGGCTCCGCGGCCGC
3'测序引物:TTGTACAAGAAAGCTGGGTCG
质粒图谱: