质粒名称:pRRLsin-SV40 T antigen-IRES-mCherry
质粒编号:58993
质粒备注:lentiviral expression of SV40 large T antigen and mCherry red fluorescent reporter
载体名称:pRRLsin.cPPT.PGK/GFP.WPRE
载体大小:6110
完整质粒大小:11405
载体类型:哺乳动物表达,慢病毒
筛选标记:mCherry red fluorescence
原核抗性:氨苄青霉素,100μg/mL
培养温度:30℃
克隆菌株:Stbl3
高拷贝/低拷贝:高拷贝
插入基因:CMV enhancer-chicken beta actin promoter
基因突变:EcoRV site in MCS converted to BamHI site
插入大小:1730
克隆方法:限制性内切酶
5'克隆位点:SalI (destroyed during cloning)
3'克隆位点:BamHI (not destroyed)
5'测序引物:none
3'测序引物:pCAG-F
质粒图谱: