质粒名称:pYLCRISPR/Cas9Pubi-H
质粒编号:66187
质粒备注:expression of Cas9 in plants, maize ubiquitin promoter (Pubi), hygro selection
载体名称:pCAMBIA1300
载体类型:Plant Expression
筛选标记:潮霉素
原核抗性:卡那霉素,50μg/mL
培养温度:37℃
克隆菌株:Top10F'
高拷贝/低拷贝:未知
插入基因:Cas9
基因突变:plant-codon optimized with higher GC contents (62.5%) in the 5’ region (400 bp) and 54.2% overall GC content
启动子:maize ubiquitin promoter (Pubi)
克隆方法:未知
5'测序引物:M13pUC-Rev
3'测序引物:NOSterm-R attgccaaatgtttgaacga
质粒图谱: