ME-180人子宫颈表皮癌细胞
产品介绍
目录号
Serial | TCHu177 | 标识符
Identifier | CSTR:19375.09.3101HUMTCHu177 | 描述
Description | 这株细胞来源于一个细胞集落不规则没有显著角质化的侵染性鳞状细胞癌。
单层培养的细胞间可以观察到带状连接，也注意到有细胞质张力丝。
1970年发现支原体污染并去除。
肿瘤坏死因子(TNF)α抑制ME-180的生长。
这株细胞含有人乳头瘤病毒(HPV)DNA，与HPV-39的同源性高于HPV-18。
在本库通过支原体检测。
在本库通过STR检测。 | 动物种别
Organism | 人 | 性别
Gender | 女 | 组织来源
Tissue and Cell Type | 宫颈表皮样癌网膜转移灶 | 形态
Morphology | 上皮细胞 | 培养基和添加剂
Complete Growth Medium and Culture Conditions | McCOY's 5A(SIGMA,货号M4892，添加NaHCO3 2.2g/L)，90%；优质胎牛血清，10%。
气相：空气，95%；二氧化碳，5%。
温度：37摄氏度。 | 供应限制
Permits and Restrictions | A。仅供研究之用。 | | REFERENCE | 21405: Sugarman BJ, et al. Recombinant human tumor necrosis factor-alpha: effects on proliferation ofnormal and transformed cells in vitro. Science 230: 943-945, 1985. PubMed: 393311122520: Sykes JA, et al. Some properties of a new epithelial cell line of human origin. J. Natl. Cancer Inst. 45:107-122, 1970. PubMed: 431773422539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors innude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 32708022995: Pater MM, Pater A. Human papillomavirus types 16 and 18 sequences in carcinoma cell lines of thecervix. Virology 145: 313-318, 1985. PubMed: 299215323180: Yee C, et al. Presence and expression of human papillomavirus sequences in human cervicalcarcinoma cell lines. Am. J. Pathol. 119: 361-366, 1985. PubMed: 299021723226: Pollack MS, et al. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor celllines. J. Natl. Cancer Inst. 66: 1003-1012, 1981. PubMed: 701721223238: Reuter S, et al. Characterization of a novel human papillomavirus DNA in the cervical carcinoma cellline ME180. J. Virol. 65: 5564-5568, 1991. PubMed: 171669423324: Scheffner M, et al. The state of the p53 and retinoblastoma genes in human cervical carcinoma celllines. Proc. Natl. Acad. Sci. USA 88: 5523-5527, 1991. PubMed: 164821829988: Hendricks DT, et al. FHIT gene expression in human ovarian, endometrial, and cervical cancer celllines. Cancer Res. 57: 2112-2115, 1997. PubMed: 918710532285: van Dijk MA, et al. A functional assay in yeas for the human estrogen receptor displays wild-type andvariant estrogen receptor messenger RNAs present in breast carcinoma. Cancer Res. 57: 3478-3485, 1997.PubMed: 927001632299: St. Geme JW, et al. Characterization of the genetic locus encoding Haemophilus influenzae type bsurface fibrils. J. Bacteriol. 178: 6281-6287, 1996. PubMed: 8892830 |
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