AE-1杂交瘤细胞抗AChE
产品介绍
目录号
Serial | HYB54 | 标识符
Identifier | CSTR:19375.09.3101ETCHYB54 | 描述
Description | 动物用纯化的人红细胞乙酰胆碱脂酶免疫。 脾细胞与gp2/O-Agl4骨髓瘤细胞融合。 产生的抗体与人及猴的乙酰胆碱脂酶结合，但反应位点与AE-2的抗原决定簇不同。 检测发现短肢畸形(鼠痘)病毒阴性。
在本库通过支原体检测。 | 动物种别
Organism | 小鼠B细胞x小鼠淋巴瘤 | 性别
Gender | *** | 组织来源
Tissue and Cell Type | B淋巴细胞,淋巴瘤 | 形态
Morphology | 淋巴母细胞 | 培养基和添加剂
Complete Growth Medium and Culture Conditions | DMEM培养基(GIBCO by life technologies ,货号C11995500BT)，90%；优质胎牛血清，10%。
气相：空气，95%；二氧化碳，5%。
温度：37摄氏度。 | 供应限制
Permits and Restrictions | A。仅供研究之用。 | | REFERENCE | Fambrough DM, Engel AG, Rosenberry TL. Acetylcholinesterase of human erythrocytes and neuromuscular junctions: homologies revealed by monoclonal antibodies. Proc Natl. Acad. Sci. USA 79:1078-1082, 1982. PubMed: 6175961 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2～d edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Centers for Disease Control (1993), Biosafi～ty in Microbiological and Biomedical Laboratories Human Health Service Publication No. (CDC) 93-8395. U.S. Dept. of Health and Human Services; 3rd Edition U.S. Government Printing Office Washington D.C. |
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