BRL 3A大鼠肝细胞
产品介绍
目录号
Serial | GNR10 | 标识符
Identifier | CSTR:19375.09.3101RATGNR10 | 描述
Description | 这个细胞株的无血清条件培养基是MSA的一个来源。 MSA多肽家族能够部分满足鸡胚成纤维细胞对血清的需求。
在本库通过支原体检测。 | 动物种别
Organism | 大鼠 | 性别
Gender | *** | 组织来源
Tissue and Cell Type | 肝 | 形态
Morphology | *** | 培养基和添加剂
Complete Growth Medium and Culture Conditions | DMEM培养基(GIBCO by life technologies ,货号C11995500BT)，90%；胎牛血清，10%。
气相：空气，95%；二氧化碳，5%。
温度：37摄氏度。 | 供应限制
Permits and Restrictions | A。仅供研究之用。 | | REFERENCE | Nissley SP , et al. Proliferation of buffalo rat liver cells in serum-free medium does not depend upon multiplication-stimulating activity (MSA). Cell 11: 441-446, 1977. PubMed: 302146Coon HG , Weiss MC . A quantitative comparison of formation of spontaneous and virus- produced viable hybrids. Proc. Natl. Acad. Sci. USA 62: 852-859, 1969. PubMed: 4308097Dulak NC , Temin HM . A partially purified polypeptide fraction from rat liver cell conditioned medium with multiplication-stimulating activity for embryo fibroblasts. J. Cell. Physiol. 81: 153-170, 1973. PubMed: 4735141Dulak NC , Shing YW . Large scale purification and further characterization of a rat liver cell conditioned medium multiplication stimulating activity. J. Cell. Physiol. 90: 127-130, 1977. PubMed: 833209Schalch DS , et al. Nonsuppressible insulin-like activity (NSILA). I. Development of a new sensitive competitive protein-binding assay for determination of serum levels. J. Clin. Endocrinol. Metab. 46: 664-671, 1978. PubMed: 755052 |
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