| In Vitro | In vitro activity: Piperine (1-Piperoylpiperidine) is the alkaloid responsible for the pungency of black pepper and long pepper, along with chavicine (an isomer of piperine). It has also been used in some forms of traditional medicine and as an insecticide. Piperine has been found to inhibit human CYP3A4 and P-glycoprotein, enzymes important for the metabolism and transport of xenobiotics and metabolites. Piperine is found to be cytotoxic towards DLA and EAC cells at a concentration of 250 μ g/ml.
Cell Assay: Standard solution is prepared by dissolving 10 mg of piperine in 100 mL
of methanol. The MTT assay is carried out to measure cell viability. Ten
thousand cells in 100 μL of DMEM media are seeded in the wells of a
96-well plate. After 24 h, existing media is removed and 100 μL of
various concentrations of piperine (20–100 μg/mL) are added and
incubated for 48 h at 37 °C in a CO2 incubator. Control cells
are supplemented with 0.05 % DMSO vehicle. At the 48th hour of
incubation, MTT (10 μL of 5 mg/mL) is added to the plate. The contents
of the plate are pipetted out carefully, the formazan crystals formed
are dissolved in 100 μL of DMSO, and the absorbance is measured at 550
nm in a microplate reader. |
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