| 包装 | 价格(元) |
| 10mM (in 1mL DMSO) | 电议 |
| 5mg | 电议 |
| 10mg | 电议 |
| 50mg | 电议 |
Cell lines | CHSE-214 cells |
Preparation Method | Control HeLa cells were pre-incubated for 1 h with 0 or 5 µg/ml Filipin Complex and Alexa Fluor 594-conjugated cholera toxin B (CTB-A594) subunit were added. After 30 min, cells were processed for confocal fluorescence microscopy. |
Reaction Conditions | 0 or 5 µg/ml Filipin Complex for 1 h |
Applications | It shows the effect of Filipin Complex on IPNV infection. No effect on viral infection was observed. |
| 产品描述 | Filipin complex is a neutral polyene,it has antifungal activity.As a fluorescent compound, Filipin complex can be used for sterol imaging in labeled biological structures,Excitation/emission values of the complexes were 338/480 nm[1，4]. Filipin III is the main component of Filipin complex which is composed of four parts[3]. The interaction of Filipin Complex with steroids was found to be related both to the functional group at the 3-position and the aliphatic chain of the steroid[7]. Cells were pretreated with 1 and 5 g/mL of Filipin Complex for 1 h and infected with IPNV at a MOI of 1 for 1 h. After 12 h of incubation in the presence of the drug, IPNV VP2/VP3 expression was revealed by immunofluorescence. It shows the effect of Filipin Complex on IPNV infection. No effect on viral infection was observed[2]. The Filipin Complex complex can bind various sterols in aqueous solutions and fungal cell membranes, especially 24α-methyl cholesterol, 24α-ethyl cholesterol, and cholesterol, inducing membrane pit formation and leakage of cell contents[5]. The Filipin Complex complex can inhibit cell growth and mitochondrial terminal electron transport in Saccharomyces cerevisiae[6]. References: |
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