| 包装 | 价格(元) |
| 1g | 电议 |
| 5g | 电议 |
Cell experiment: | F. tularensis is cultured in TSBc supplemented with 44 μM Resazurin sodium salt at 37℃ with shaking for 24 h. At select time points, a Spectronic 200 Spectrophotometer is used to measure the absorbance at 600 nm and 570 nm to detect the presence of Resazurin sodium salt and resorufin, respectively. The ratio of these two optical densities is used to evaluate reduction of Resazurin sodium salt to resorufin over time[1]. |
| 产品描述 | The non-fluorescent resazurin has been used as a fluorogenic oxidation-reduction indicator in various cells by flow cytometry, fluorescence microscopy and high-throughput screening. Its red-fluorescent product, resorufin, has absorption/emission maxima ~575/585 nm. In vitro: Resazurin has been applied as a simple and versatile way to measure cell proliferation and cytotoxicity. Although resazurin presented plenty of advantages over other cytotoxicity or proliferation tests, everal drawbacks to the routine use of resazurin were also observed. Previoius studies showed accumulation of the fluorescent product of resazurin in the medium which could result in an overestimation of cell population. Moreover, the extensive reduction of resazurin by metabolically active cells would lead to a final nonfluorescent product, and thus an underestimation of cellular activity [1]. In order to evaluate the effect of resazurin over longer incubation times, MCF7, MCF10A, 3T3-L1 and D1 cell lines were tested. Results showed that MCF7, 3T3-L1, and D1 cells cultured for 8 days with 20% resazurin had significantly less cell survivability. Moreover, levels of metabolic activity, DNA concentration, as well as glucose consumption of the cell lines in medium with 20% resazurin were significantly lower. Data also showed that the toxicities were more pronounced in the cancer cell lines than in the noncancerous cells [2]. References: |
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